anti darp 32  (Cell Signaling Technology Inc)

Journal: The Journal of Neuroscience

Article Title: Consolidation of Goal-Directed Action Depends on MAPK/ERK Signaling in Rodent Prelimbic Cortex

doi: 10.1523/JNEUROSCI.1772-16.2016

Figure Lengend Snippet: Assessment of pERK activity in pDMS-projecting neurons and pERK expression in the pDMS 5 and 60 min after instrumental or yoked training. A–D, Mean percentage of FG-labeled neurons colabeled with pERK per slice in layers 2–3 (A) and layers 5–6 (B) of the posterior PL and layers 2–3 (C) and layers 5–6 (D) of the anterior PL. Error bars indicate SEM. E, Representative pDMS section (coronal 30 μm section) from one rat showing pERK expression (green) and DARP-32 stained MSNs (red). Scale bar, 100 μm. F, Mean number of pERK-expressing MSNs per slice in the pDMS for each group. Error bars indicate SEM. *p < 0.05.

Article Snippet: The immunofluorescence protocol used for staining pERK in the pDMS was identical to that used for the PL, except that in addition to the rabbit anti-phospho-p44/42 MAPK (1:300), a purified mouse anti-DARP-32 (1:1000, cat #611520; BD Biosciences, RRID: AB_398980) was applied.

Techniques: Activity Assay, Expressing, Labeling, Staining